Resonance CARS Study of the Structure of “Green” and “Red” Chromophores within the Red Fluorescent Protein DsRed
- 23 August 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 124 (37) , 10992-10993
- https://doi.org/10.1021/ja0260824
Abstract
Vibrational spectra of red fluorescent protein DsRed have been studied for the first time by polarization-sensitive multiplex coherent anti-Stokes Raman scattering at two excitation wavelengths, 545 and 583 nm, in resonance with the absorption bands of the immature “green” and mature “red” protein chromophores. Overall vibrational patterns of both DsRed chromophores were found to be similar to each other and to differ from that of S65T-GFP at pH8. The combined analysis of our CARS data and published structural information suggest that both “green” and “red” DsRed species possess an extended chromophore structure. Consequently, our data suggest that π-bonding system extension during isomerization around the cis peptide bond between Phe 65 and Gln 66 is a necessary but not sufficient step in DsRed chromophore maturation.Keywords
This publication has 9 references indexed in Scilit:
- Vibrational bands of luminescent zinc(II)‐octaethylporphyrin using a polarization‐sensitive ‘microscopic’ multiplex CARS techniqueJournal of Raman Spectroscopy, 2001
- Resonance Raman Scattering by the Green Fluorescent Protein and an Analogue of Its ChromophoreThe Journal of Physical Chemistry B, 2001
- Red Fluorescent Protein from Discosoma as a Fusion Tag and a Partner for Fluorescence Resonance Energy TransferBiochemistry, 2001
- Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolutionProceedings of the National Academy of Sciences, 2001
- Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine)Proceedings of the National Academy of Sciences, 2000
- The structure of the chromophore within DsRed, a red fluorescent protein from coralProceedings of the National Academy of Sciences, 2000
- EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two‐photon and fluorescence lifetime microscopyFEBS Letters, 2000
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- On the coherent vibrational phase in polarization sensitive resonance CARS spectroscopy of copper tetraphenylporphyrinThe Journal of Chemical Physics, 1997