Kpn I family of long-dispersed repeated DNA sequences of man: evidence for entry into genomic DNA of DNA copies of poly(A)-terminated Kpn I RNAs.

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Abstract
Eight c[complementary]DNA clones were isolated complementary to the human Kpn I repeat and the base sequence of 3 was determined. A portion of the base sequences of 3 human Kpn I family members was determined. The 3 cDNA sequences are extensively homologous with the 3'' ends of the 3 genomic Kpn I family members and with a simian Kpn I family member recently described. The genomic repeats terminate in regions of sequence rich in dAMP residues close to sequences at the 3'' ends of the cDNA clones; a precise 3''-terminal nucleotide cannot be distinguished. These structural features are consistent with the dispersal of at least some Kpn I family members by entry into genomic DNA of copies of Kpn I RNA transcripts. Each cDNA contains a long poly(dAMP) homopolymer at its 3'' end and either 1 or 2 A-A-T-A-A-A polyadenylylation signal sequences upstream from it, suggesting that Kpn I family members may be transcribed by RNA polymerase II.