Applicability of a “Speed” Congenic Strategy to Dissect Blood Pressure Quantitative Trait Loci on Rat Chromosome 2
- 1 January 2000
- journal article
- other
- Published by Wolters Kluwer Health in Hypertension
- Vol. 35 (1) , 179-187
- https://doi.org/10.1161/01.hyp.35.1.179
Abstract
—The identification of any quantitative trait locus (QTL) via a genome scan is only the first step toward the ultimate goal of gene identification. The next step is the production of congenic strains by which the existence of a QTL may be verified and the implicated chromosomal region be reduced to a size applicable to positional cloning of the causal gene. We used a speed congenic breeding protocol previously verified in mice for 2 blood pressure QTLs on rat chromosome 2. Four congenic strains were produced through introgression of various segments of chromosome 2 from Wistar-Kyoto rats from Glasgow colonies [WKY(Gla) rats] into the recipient stroke-prone spontaneously hypertensive rats from Glasgow colonies [SHRSP(Gla)], and vice versa. The number of backcross generations required for each strain to achieve complete homozygosity at 83 background genetic markers in a “best” male varied between 3 and 4. Transfer of the region of rat chromosome 2 containing both QTLs from WKY(Gla) into an SHRSP(Gla...Keywords
This publication has 21 references indexed in Scilit:
- Linkage Analysis and Construction of a Congenic Strain for a Blood Pressure QTL on Rat Chromosome 9Genomics, 1998
- An integrated genetic linkage map of the laboratory ratMammalian Genome, 1998
- Theoretical and empirical issues for marker-assisted breeding of congenic mouse strainsNature Genetics, 1997
- Speed congenics: a classic technique in the fast lane (relatively speaking)Immunology Today, 1997
- Sensitivity to cerebral ischaemic insult in a rat model of stroke is determined by a single genetic locusNature Genetics, 1997
- Genetic isolation of a region of chromosome 8 that exerts major effects on blood pressure and cardiac mass in the spontaneously hypertensive rat.Journal of Clinical Investigation, 1997
- Effects of renin gene transfer on blood pressure and renin gene expression in a congenic strain of Dahl salt-resistant rats.Journal of Clinical Investigation, 1996
- Genetic Dissection of Complex TraitsScience, 1994
- Chromosomal mapping of two genetic loci associated with blood-pressure regulation in hereditary hypertensive ratsNature, 1991
- Genetic mapping of a gene causing hypertension in the stroke-prone spontaneously hypertensive ratCell, 1991