Methylation of desmethyl analogue of Y nucleosides. Wyosine from guanosine
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (15) , 5243-6255
- https://doi.org/10.1093/nar/11.15.5243
Abstract
Wyoslne la. one of the fluorescent hypermodified Y nucleosides found In tRNAsPhe, was synthesized chemically from its biogenetic precursor guanosine 2. The route involved transformation of 2. into the tricyclic structure 3a and subsequent methylation at N-4. The major products of various methylation procedures were isomers of wyosine, methylated at H-5 (5b) or at H-l (4). Mesoionic compound 4 is a new analogue of 7-methylguanosine 5, modified nucl-eoside occuring in the unique positions in transfer, messenger and ribosomal HNAs. The chromatographio and spectral ohaxacteristios of wyosine and its isomers is given.Keywords
This publication has 12 references indexed in Scilit:
- Ribosome structure. Localization of 7-methylguanosine in the small subunits of Escherichia coli and chloroplast ribosomes by immunoelectron microscopy.Journal of Biological Chemistry, 1982
- Proton nuclear magnetic resonance of minor nucleosides in yeast phenylalanine transfer ribonucleic acid. Conformational changes as a consquence of aminoacylation, removal of the Y base, and codon-anticodon interactionBiochemistry, 1979
- Isolation of hydroxy-Y base from rat liver tRNAPheNucleic Acids Research, 1979
- Incorporation of lysine into Y base of phenylalanine tRNA in Vero cellsNucleic Acids Research, 1979
- Guanosine-methyldiazonium ion reactionBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
- Capping of eucaryotic mRNAsCell, 1976
- Chemical transformations of 7,9-disubstituted purines and related heterocycles. Selective reduction of imines and immonium saltsThe Journal of Organic Chemistry, 1976
- Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acidBiochemistry, 1976
- Origin of the nucleoside Y in yeast tRNAPheFEBS Letters, 1973
- The alkylation of 2′-deoxyguanosine and of thymidine with diazoalkanes. Some observations on O-alkylationBiochemical Journal, 1973