Density Gradient Centrifugation Studies on Rabies Virus

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Abstract
Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski . Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92: 102–106. 1966.—Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained by rate zonal centrifugation in preformed sucrose gradients, it was possible to calculate a sedimentation coefficient of about 600 S for rabies virus. Sedimentation coefficients of about 23 S and 10 S were calculated for two soluble rabies antigens present in infected tissue-culture fluids, and they showed a density of 1.26 g/ml in cesium chloride solutions.