Determination of apoplastic K+in intact leaves by ratio imaging of PBFI fluorescence
Open Access
- 1 August 1997
- journal article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 48 (8) , 1609-1614
- https://doi.org/10.1093/jxb/48.8.1609
Abstract
The tetraammonium salt of the K+ binding fluorescent dye benzofuran isophthalate (PBFI) was used to investigate the influence of potassium nutrition (0.1–2.1 mol m−3) on apoplastic K+ inVicia faba leaves by means of ratio imaging. As a reference the infiltration-centrifugation method was used. Both methods reflected the influence of K+ supply on apoplastic K+ concentration. The abaxial leaf side revealed significantly higher K+ concentrations (20-25 mol m−3) than the adaxial side (5–8 mol m−3). Application of CCCP led to an immediate increase in apoplastic K+ demonstrating the reliability of the PBFI method.Keywords
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