1H nuclear-magnetic-resonance spectra of the methyl group of the acetamido moiety and the structure of acid glycosaminoglycans in solution

Publisher Website
Google Scholar
Abstract
1H resonances of the methyl group in the acetamido moiety of several types of glycosaminoglycans are reported at 300 MHz in 2H2O. Dermatan sulfates with various L-iduronate/D-glucuronate ratios are compared with chrondroitin sulfates with various contents and positions of sulfate ester substitution. Hyaluronate oligomers are compared with 2-acetamido-2-deoxy-D-glucose, with heparin sulfate and keratan sulfate. The major determinant of the chemical shift of the acetamido methyl resonance in closeness of approach between carboxy groups and the acetamido group, in agreement with chemical evidence based on periodate-oxidation kinetics.