THE EFFECT OF SPORULATION TEMPERATURE ON THE THERMAL RESISTANCE OF BACILLUS COAGULANS VAR. THERMOACIDURANS

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Abstract
The effect of sporulation temperature on the thermal resistance of B. coagulans var. thermoacidurans was determined by producing spore crops in duplicate at different growth temperatures. Duplicate thermal death rate determinations were made of each spore harvest. The data were treated statistically to compute and compare the thermal death rate constants obtained for each growth temperature. Higher growth temperatures markedly enhanced the thermal resistance of the spores produced. Phantom thermal death time curves were then established and the z values statistically computed and compared for spore harvests of the highest versus those of the lowest thermal death rate. No significant difference was found in the z value between the 2 types of spore suspensions. This finding permits the extrapolation of results obtained from different heating conditions. The results emphasized the necessity of adequate replication, not only of the thermal death runs but also of the spore harvests produced under the same conditions. The deviation of some points from the inherent linearity of the survivor curve was also discussed.

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