Adsorption of Human Alpha (Leukocyte) Interferon on Glass: Contributions of Electrostatic and Hydrophobic Forces
- 1 January 1982
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 2 (2) , 229-234
- https://doi.org/10.1089/jir.1982.2.229
Abstract
Human leukocyte-derived interferon (HuIFN-α) can be displaced from controled-pore glass (CPG) with alkylamines at 0.1–0.3 M concentration. The eluting power of an alkylamine correlates positively with the extent of its alkylation: CH3NH3 + < (CH3)2NH2 + < (CH3)3NH+ < (CH3)4N+. Interferon can also be recovered using an electrolyte and ethylene glycol as cosolvents. Apparently, both hydrophobic and electrostatic forces are involved in the adsorption of HuIFN-α to CPG.Keywords
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