An improved procedure for the purification of plasma membranes from Dictyostelium discoideum
- 1 December 1977
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry
- Vol. 55 (12) , 1233-1236
- https://doi.org/10.1139/o77-184
Abstract
A novel procedure was recently described for the purification of plasma membranes of D. discoideum. Considerable enrichment of plasma membrane marker enzymes was achieved, but since purified mitochondrial and endoplamic reticulum fractions were unavailable, it was not possible to accurately assess the contamination level of these organelles. The plasma membrane preparation procedure was slightly modified improving purification, and purified mitochondrial and endoplasmic reticulum fractions were prepared. The data suggested that the contamination of the plasma membranes by endoplasmic reticulum membranes was no < 10%, and probably considerably less. No mitochondrial contamination was detectable.This publication has 2 references indexed in Scilit:
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966
- Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic StudiesJournal of Biological Chemistry, 1962