Liquid-Phase Synthesis of Naturally Occurring Peptides, I. Syntheses of Leucine-Enkephalin and Methionine-Enkephalin on a p-Alkoxybenzyl-Modified Soluble Support
- 1 January 1981
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 362 (2) , 1385-1392
- https://doi.org/10.1515/bchm2.1981.362.2.1385
Abstract
The liquid-phase synthesis of 2 pentapeptides corresponding to the amino acid sequences of Leu- and Met-enkephalin is described. Modified monofunctional poly(ethylene glycol) containing a p-alkoxybenzyl alcohol functional group was employed as the soluble polymeric support. Cleavage of the peptides from the polymer, and the removal of protecting groups, was achieved with trifluoroacetic acid at room temperature. The free peptides were purified by column chromatography on DEAE-Sephadex, and were identical in both physical and biological characteristics with reference material. The proposed support is effective for a mild cleavage of peptides from the soluble polymer during liquid-phase synthesis.This publication has 10 references indexed in Scilit:
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