Structure of the snake short‐chain neurotoxin, erabutoxin c, precursor gene
Open Access
- 1 November 1990
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 193 (3) , 629-633
- https://doi.org/10.1111/j.1432-1033.1990.tb19380.x
Abstract
A 2.4-kb genomic clone, which encodes the precursor of a snake neurotoxin, erabutoxin c, was isolated from the liver of the sea snake, Laticauda semifasciata. The erabutoxin c gene is about 1.2 kb long and consists of three exons and two introns. The first intron is found at the position corresponding to the signal peptide between amino acid residues 18(Leu) and 19(Gly). The second intron is located at the position corresponding to the central loop of the mature toxin, between amino acid residues 33(Arg) and 34(Gly). A TATA box consensus sequence, characteristic of promoter regions, is identified 29–33 nucleotides upstream from the transcription-initiation site identified by nuclease S1 analysis. The erabutoxin c cDNA nucleotide sequence, deduced from the present work, is compared with the cDNA sequences of erabutoxins a and b reported previously. Replacements are found at three positions, two of which correspond to the amino acid replacements found among the toxins, while the other is silent.Keywords
This publication has 19 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- [15] Isolation of genomic DNAPublished by Elsevier ,1987
- Current view on the structure-function relationship of postsynaptic neurotoxins from snake venomsPharmacology & Therapeutics, 1987
- Erabutoxin bEuropean Journal of Biochemistry, 1985
- Cloning and sequence analysis of the cDNA encoding a snake neurotoxin precursorBiochimie, 1985
- Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitroCell, 1984
- A recovered avian myelocytomatosis virus that induces lymphomas in chickens: pathogenic properties and their molecular basisCell, 1983
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982
- Organization and Expression of Eucaryotic Split Genes Coding for ProteinsAnnual Review of Biochemistry, 1981
- Erabutoxins a, b and c in sea snake Laticauda semifasciata venomToxicon, 1973