The Effect of Estradiol on RNA Synthesis in Preimplantation Mouse Embryos Cultured in vitro

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Abstract
The effect of concentrations of 17-.beta.-estradiol from 10-5-10-9 M, on RNA synthesis in preimplantation mouse embryos cultured in vitro, was probed. Blastocysts incubated with 3H-uridine and hormone simultaneously, from 0.5-24 h, showed no major stimulation or inhibition of RNA synthesis. The differential sensitivity of 8 cell and blastocyst embryos to estradiol was probed. Preincubation with 10-5-10-9 M estradiol for 21 h, followed by a 3 h incubation with 3H-uridine, also showed no major stimulation or inhibition of RNA synthesis in either 8 cell or blastocyst embryos. RNA synthesis in 8 cell and blastocyst mouse embryos was refractory to modulation by exogenously added estradiol. Several explanations are put forth to account for these results.