Expression of reverse transcriptase activity of human T-lymphotropic virus type III (HTLV-III/LAV) in Escherichia coli
- 1 September 1986
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 59 (3) , 743-745
- https://doi.org/10.1128/jvi.59.3.743-745.1986
Abstract
The pol gene from a biologically active clone of the human T-cell lymphotropic virus type III provirus was inserted into a bacterial expression vector. The resulting gene fusion induced the formation of active reverse transcriptase that could be readily detected in extracts of bacterial cells. The activity exhibited the template and divalent cation requirements of the authentic enzyme. These constructs will be useful for safe and rapid analysis of potential inhibitors of this important enzyme.This publication has 17 references indexed in Scilit:
- Characterization of the AIDS-associated retrovirus reverse transcriptase and optimal conditions for its detection in virionsVirology, 1985
- Complete nucleotide sequence of the AIDS virus, HTLV-IIINature, 1985
- RIBAVIRIN SUPPRESSES REPLICATION OF LYMPHADENOPATHY-ASSOCIATED VIRUS IN CULTURES OF HUMAN ADULT T LYMPHOCYTESThe Lancet, 1984
- Suramin Protection of T Cells in Vitro Against Infectivity and Cytopathic Effect of HTLV-IIIScience, 1984
- Frequent Detection and Isolation of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and at Risk for AIDSScience, 1984
- Characterization of the RNA dependent DNA polymerase of a new human T lymphotropic retrovirus (lymphadenopathy associated virus)Biochemical and Biophysical Research Communications, 1984
- Isolation of Human T-Cell Leukemia Virus in Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- Suramin: A potent inhibitor of the reverse transcriptase of RNA tumor virusesCancer Letters, 1979
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976