Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs.

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Abstract
Three hamster monoclonal antibodies (mAbs), all recognizing different epitopes present on the native form of the murine T-cell antigen receptor (TCR) .gamma..delta. subunits, have been generated. mAb 3A10 is specific to a pan-murine TCR .gamma..delta., recognizing a C.delta. constant region determinant. mAb 8D6 is specific to a subset of T cells expressing V.gamma.4- and V.delta.5-encoded .gamma..delta.TCR, and mAb 5C10 is clonotypic. Using these and other mAbs directed against a variety of T-cell surface markers, we quantitated and characterized .gamma..delta. T cells present in developing thymuses as well as in the conventional lymphatic organs by flow cytometry. These studies revealed that (i) many .gamma..delta. thymocytes and peripheral T cells bear CD4 and/or CD8 molecules, [ii) T cells bearing both .alpha..beta. and .gamma..delta. TCRs are scarce, and (iii) thymocyte subsets bearing TCR .gamma..delta. encoded by different combinations of V.gamma. and V.delta. gene segments appear in waves during ontogeny.