An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria
- 1 February 1997
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (3) , 675-676
- https://doi.org/10.1093/nar/25.3.675
Abstract
A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.Keywords
This publication has 6 references indexed in Scilit:
- Detection of viableVibrio cholerae by reverse-transcriptase polymerase chain reaction (RT-PCR)Molecular Biotechnology, 1996
- A rapid protocol for isolation of RNA from mycobacteriaLetters in Applied Microbiology, 1995
- Isolation of RNA from Mycobacterium tuberculosis using a nitrogen decompression chamber.1995
- An efficient and high-yielding method for isolation of RNA from mycobacteria.1994
- A Method to Isolate RNA from Gram-Positive Bacteria and MycobacteriaAnalytical Biochemistry, 1994
- Structure of Mycobacteria: Recent Developments in Defining Cell Wall Carbohydrates and ProteinsClinical Infectious Diseases, 1989