SHORT COMMUNICATION: Inhibition of rat mammary tumorigenesis by dietary cholesterol

Abstract
The effects of dietary cholesterol and oxidized cholesterol on mammary tumor development were examined in female Sprague-Dawley rats exposed to the carcinogen N-methyl-N-nitrosourea (MNU). Animals were administered 50 mg/kg MNU at 50 days of age and fed either a control (AIN-76) diet or the control diet supplemented with 0.3% cholesterol or 0.3% oxidized cholesterol for up to 26 weeks. The oxidized cholesterol was prepared by heating cholesterol at 110°C for 48 h. Gas chromatographic analysis of the oxidized cholesterol revealed a 2% yield of oxidation products in addition to a large amount of unchanged cholesterol (>96%). Tumor incidence in the cholesterol group (67%) was significantly lower than in the control group (96%, P P P de novo cholesterol synthesis in preneoplastic and/or tumor cells, thereby inhibiting their proliferation.

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