FLUORESCENT STAINING OF NUCLEAR-ENVELOPE COATED WITH HEPARIN
- 1 January 1980
- journal article
- research article
- Vol. 83 (1) , 49-54
Abstract
Naturally and artificially devitalized cells of various origin [mouse macrophage, chicken erythrocyte and human erythrocyte] can be loaded with heparin and subsequently stained with DAPI, a newer fluorochrome (4'',6-diaminidino-2-phenylindole), leading to a bright yellow fluorescence of the nuclear envelope. Depending on the cell type it is also possible to coat the outer cell membrane with heparin or other sulfated mucopolysaccharides and to achieve similar staining phenomena with DAPI.This publication has 8 references indexed in Scilit:
- Uptake and degradation of mast-cell granules by mouse peritoneal macrophagesBiochemical Journal, 1979
- Effects of DAPI on human leukocytes in vitroCytogenetic and Genome Research, 1979
- Increased uptake and desulphaton of heparin by mouse macrophages in the presence of polycationsBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Uptake and intracellular distribution of neutral red in cultured fibroblastsExperimental Cell Research, 1978
- Immunologic Release of Heparin from Purified Rat Peritoneal Mast CellsThe Journal of Immunology, 1977
- DAPI fluorescence of plant chromosomes prestained with actinomycin DExperimental Cell Research, 1976
- Desulphation of heparin by mice and guinea pig leukocytesBiochimica et Biophysica Acta (BBA) - General Subjects, 1976
- Phagocytosis of granules from disrupted mast cellsThe Anatomical Record, 1958