GROWTH-HORMONE STIMULATION OF NORMAL AND LEUKEMIC HUMAN LYMPHOCYTE-T PROLIFERATION INVITRO

1 January 1981

journal article
research article

Vol. 58 (2) , 337-340

Abstract

The effect of human growth hormone on T lymphocytes was studied using a clonogenic assay for mitogen-responsive human peripheral blood T lymphocytes. Lymphocytes were purified by density gradient centrifugation and incubated in the presence of phytohemagglutinin using a 2-layer agar technique for CFU-T-lymphocyte culture. Nanogram concentrations of human growth hormone, ovine prolactin, human chorionic somatomammotropin or growth hormone fragment were added to cell cultures. Growth hormone potentiated normal T-cell colony formation in a species-specific manner. Cells from a homogeneous T-lymphoblast line derived from a patient with a T-cell variant of hairy cell leukemia also showed augmentation of colony growth in the presence of human growth hormone. These studies provide evidence for a direct effect of growth hormone on normal and some neoplastic human T cells.

This publication has 3 references indexed in Scilit:

Growth Factors
Annals of Internal Medicine, 1980
Effect of polypeptide hormones on stimulation of casein secretion by mouse mammary epithelial cells grown on floating collagen gels
In Vitro Cellular & Developmental Biology - Plant, 1978
T-Lymphocyte Variant of Hairy-Cell Leukemia
Annals of Internal Medicine, 1978