α-Lactalbumin Production in Human Mammary Carcinoma

14 November 1975

journal article
research article
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 190 (4215) , 673-675
https://doi.org/10.1126/science.1188362

Abstract

Alpha-Lactalbumin was isolated from mature human milk and utilized as an immunogen in rabbits. A radioimmunoassay was developed that was capable of detecting nanogram quantities of the antigen. Alpha-Lactalbumin synthesis was detected in human breast cancer cells (MCF-7) cultivated as a continuous cell line in vitro. Other human carcinoma epithelial cell lines (throat and cervix) failed to react in this assay. The ability to synthesize alpha-lactalbumin by breast carcinoma cells appeared to be independent of the addition of prolactin to the culture medium.

Keywords

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