PGI2 enhanced cAMP content in bovine coronary arteries in the presence of isobutylmethylxanthine

1 August 1979

journal article
research article
Published by Springer Nature in Naunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie

Vol. 308 (2) , 107-110
https://doi.org/10.1007/bf00499051

Abstract

Summary Incubation of bovine coronary artery (BCA) rings with isobutylmethylxanthine (IBMX) resulted in a time-dependent increase of cAMP content. This effect was blocked, when the rings were preincubated with indomethacin or 15-hydroperoxy-arachidonic acid for 5 min, indicating that the IBMX-induced increase in cAMP content may depend on endogenous PGI₂ formation. PGE₂ did not increase the cAMP content in BCA rings. Dipyridamole did not affect cAMP content, when used as a substitute for IBMX. It is suggested that PGI₂ stimulates cAMP formation in arterial walls, but that this effect only becomes visible in the presence of a phosphodiesterase inhibitor.

Keywords

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