The 5' untranslated region of the human γ‐glutamyl transferase mRNA contains a tissue‐specific active translational enhancer
- 11 October 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 332 (1-2) , 88-92
- https://doi.org/10.1016/0014-5793(93)80490-l
Abstract
We report the functional and structural analysis of the 5' untranslated region (5'UTR) of human hepatoma HepG2 γ-glutamyltransferase (GGT) mRNA. Transient expression of a hybrid GGT-luciferase gene in HepG2, MIA-Pa-Ca-2 and MG 63 cell lines shows that this 5'UTR acts as a tissue-specific translational enhancer. Evidence for transcripts with multiple 5'UTR coding for HepG2 GGT was obtained by RNase protection. Computer analysis of this 5'UTR detected the existence of a stable stem and loop structure containing multiple steroid modulatory elements.Keywords
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