Enhancement of murine macrophage binding of and response to bacterial lipopolysaccharide (LPS) by LPS-binding protein

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Abstract
We have studied the effects of highly purified rabbit lipopolysaccharide (LPS)-binding protein (LBP) on the ability of murine bone marrow-derived macrophages to respond to bacterial LPS. Macrophage responses studied include the secretion of tumor necrosis factorα, production of arginine-derived nitrite (NO2−), and killing of an intracellular pathogen, Leishmania enriettii. Macrophages from either CBA or LPS-hyporesponsive C3H/HeJ mice exhibited significantly greater sensitivity to LPS in the presence of LBP Furthermore, both CBA and C3H/HeJ macrophages demonstrated an LBP-depen- dent enhancement of LPS binding. These results suggest that C3H/HeJ macrophages are capable of binding LPS- LBP complexes and support the hypothesis that hypo- responsiveness in this strain involves a step subsequent to LPS binding. Furthermore, these findings provide additional evidence of the important role played by the acute- phase plasma protein LBP in modifying host response to LPS.

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