A new and versatile method for the successful conversion of AFLPTM markers into simple single locus markers
Open Access
- 15 May 2003
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 31 (10) , 55e-55
- https://doi.org/10.1093/nar/gng055
Abstract
Genetic markers can efficiently be obtained by using amplified fragment length polymorphism (AFLP) fingerprinting because no prior information on DNA sequence is required. However, the conversion of AFLP markers from complex fingerprints into simple single locus assays is perceived as problematic because DNA sequence information is required for the design of new locus‐specific PCR primers. In addition, single locus polymorphism (SNP) information is required to design an allele‐specific assay. This paper describes a new and versatile method for the conversion of AFLP markers into simple assays. The protocol presented in this paper offers solutions for frequently occurring pitfalls and describes a procedure for the identification of the SNP responsible for the AFLP. By following this approach, a high success rate for the conversion of AFLP markers into locus‐specific markers was obtained.Keywords
This publication has 17 references indexed in Scilit:
- Population genetics of the yellow fever mosquito in Trinidad: comparisons of amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) markersMolecular Ecology, 1999
- dCAPS, a simple technique for the genetic analysis of single nucleotide polymorphisms: experimental applications in Arabidopsis thaliana geneticsThe Plant Journal, 1998
- Chapter 12 Mapping Zebrafish Mutations by AFLPPublished by Elsevier ,1998
- PCR-based fingerprinting using AFLPs as a tool for studying genetic relationships in Lactuca spp.Theoretical and Applied Genetics, 1996
- A high-resolution map of the vicinity of the R1 locus on chromosome V of potato based on RFLP and AFLP markersMolecular Genetics and Genomics, 1995
- Combined mapping of AFLP and RFLP markers in barleyMolecular Genetics and Genomics, 1995
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995
- A procedure for mapping Arabidopsis mutations using co‐dominant ecotype‐specific PCR‐based markersThe Plant Journal, 1993
- A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications.1993
- Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuceTheoretical and Applied Genetics, 1993