Partial purification and characterization of cyclic nucleotide phosphodiesterases from human bronchial tissue
- 1 October 1978
- journal article
- research article
- Published by Springer Nature in Molecular and Cellular Biochemistry
- Vol. 21 (1) , 9-15
- https://doi.org/10.1007/bf00230191
Abstract
DEAE-Sephadex chromatography reveals the presence in extracts of human bronchial tissue of at least three separate cyclic nucleotide phosphodiesterases: a cyclic GMP-specific high affinity enzyme, a non-specific low affinity enzyme, and a high affinity cyclic AMP-specific enzyme. The activity of each fraction was partially characterized with respect to kinetic parameters, thermal stability, and the influence of a number of inhibitors. Each activity was found to resemble the activity of the previously characterized corresponding enzyme from whole lung tissue extracts. A high affinity non-specific phospho-diesterase previously isolated from lung tissue is missing in extracts of bronchial tissue.Keywords
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