Transcellular transport of fluorescein dextran through an arterial endothelial cell monolayer.
- 1 January 1986
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 11 (4) , 343-349
- https://doi.org/10.1247/csf.11.343
Abstract
Transcellular transport of fluorescein dextran (FD) of various molecular weights (4K, 10K, 20K, 70K and 150K daltons) through porcine arterial endothelial cells cultured on a type I collagen gel supported by a dacron sheet was studied and compared with the transport of low density lipoprotein labeled with rhodamine B (RB-LDL) described previously (Hashida et al., Cell Struct. Funct. 11, 31-42, 1986). The rate of FD transport through the monolayer depended on the size of the FD. FD transport was not temperature-dependent and was not a saturable process. Our findings show that FD transport differs from RB-LDL transport which is temperature- and dose-dependent. The mechanism of the transport of FD is compared with that of RB-LDL.This publication has 1 reference indexed in Scilit:
- Open junctions in the endothelium of the postcapillary venules of the diaphragm.The Journal of cell biology, 1978