Phosphoryl Transfer and Calcium Ion Occlusion in the Calcium Pump
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- 11 June 2004
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 304 (5677) , 1672-1675
- https://doi.org/10.1126/science.1099366
Abstract
A tight coupling between adenosine triphosphate (ATP) hydrolysis and vectorial ion transport has to be maintained by ATP-consuming ion pumps. We report two crystal structures of Ca2+-bound sarco(endo)plasmic reticulum Ca2+–adenosine triphosphatase (SERCA) at 2.6 and 2.9 angstrom resolution in complex with (i) a nonhydrolyzable ATP analog [adenosine (β–γ methylene)–triphosphate] and (ii) adenosine diphosphate plus aluminum fluoride. SERCA reacts with ATP by an associative mechanism mediated by two Mg2+ ions to form an aspartyl-phosphorylated intermediate state (Ca2-E1∼P). The conformational changes that accompany the reaction with ATP pull the transmembrane helices 1 and 2 and close a cytosolic entrance for Ca2+, thereby preventing backflow before Ca2+ is released on the other side of the membrane.Keywords
This publication has 23 references indexed in Scilit:
- Importance of Transmembrane Segment M1 of the Sarcoplasmic Reticulum Ca2+-ATPase in Ca2+ Occlusion and Phosphoenzyme ProcessingPublished by Elsevier ,2004
- Importance of Conserved N-domain Residues Thr441, Glu442, Lys515, Arg560, and Leu562 of Sarcoplasmic Reticulum Ca2+-ATPase for MgATP Binding and Subsequent Catalytic StepsJournal of Biological Chemistry, 2003
- A structural model for the catalytic cycle of Ca2+-ATPaseJournal of Molecular Biology, 2002
- Structural organization, ion transport, and energy transduction of P-type ATPasesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1996
- Ca2+ Binding to Occluded Sites in the CrATP-ATPase Complex of Sarcoplasmic Reticulum: Evidence for Two Independent High-Affinity SitesBiochemistry, 1994
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Kinetics of calcium dissociation from its high-affinity transport sites on sarcoplasmic reticulum ATPaseBiochemistry, 1991
- Location of high affinity Ca2 +-binding sites within the predicted transmembrahe domain of the sarco-plasmic reticulum Ca2+-ATPaseNature, 1989
- Occlusion of Divalent Cations in the Phosphorylated Calcium Pump of Sarcoplasmic ReticulumEuropean Journal of Biochemistry, 1980
- Partial reactions in the catalytic and transport cycle of sarcoplasmic reticulum ATPaseBiochemistry, 1978