Physiological properties of Azospirillum brasilense sp 7 in a malate limited chemostat.

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Abstract
A. brasilense Sp 7 was grown in a N-free medium in a chemostat with malate as the sole C source. Several steady states were established. Malate limitation occurred at dilution rates < 0.05 h-1. Maximum growth rate and Ks [half-saturation coefficient] were calculated from Monod''s model. Poly-.beta.-hydroxybutyrate content decreased under malate limitation from 18.5% to .apprx. 2%. Protein exudation increased under these conditions. The acetylene reduction assay was not suitable for this continuous culture system. A model for the physiological properties of A. brasilense in a malate limited chemostat was developed.