Mycoplasma granularum of Swine Origin as a Tissue Culture Contaminant

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Abstract
Summary A mycoplasma isolated from murine leukemia cells in culture was found to be serologically similar to a mycoplasma strain recovered from the nasal cavity of a pig. Additional serological tests revealed both strains to be Mycoplasma granularum, a species previously identified only in swine hosts. Tissue culture contamination with swine mycoplasma reemphasizes control problems in maintaining uncontaminated cell lines and poses major questions about sources of these strains in cell cultures.

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