Bioaffinity Sensor Using Synthetic Oligonucleotide-Modified Au Electrode.

1 January 1997

journal article
Published by Springer Nature in Analytical Sciences

Vol. 13 (Supplement) , 455-456
https://doi.org/10.2116/analsci.13.supplement_455

Abstract

A 20-mer oligonucleotide whose 5′-end phosphate was modified with ω-thiohexyl group was applied in order to modify a Au electrode with its duplex form. Cyclic voltammograms of ferrocyanide/ferricyanide redox couple with the DNA-immobilized electrode gave the peak currents due to the reversible electrode reaction, which were significantly enhanced on adding acridine orange (AO). The cathodic peak current showed almost a linear relationship with AO concentration in the range of 10^-7-7×10^-7M, and then saturated beyond the concentration of 10^-6M. From the concentration-dependent profiles, an apparent binding constant of (9.1±5.4)×10⁵M^-1 was obtained for the AO-DNA system. The obtained data agreed fairly well with the reported value (1.2±0.3)×10⁶M^-1.

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