Relative quantification of mRNA: comparison of methods currently used for real-time PCR data analysis
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Open Access
- 1 January 2007
- journal article
- research article
- Published by Springer Nature in BMC Molecular Biology
- Vol. 8 (1) , 113
- https://doi.org/10.1186/1471-2199-8-113
Abstract
Fluorescent data obtained from real-time PCR must be processed by some method of data analysis to obtain the relative quantity of target mRNA. The method chosen for data analysis can strongly influence results of the quantification.Keywords
This publication has 21 references indexed in Scilit:
- Quantitative real-time RT-PCR data analysis: current concepts and the novel “gene expression’s C T difference” formulaJournal of Molecular Medicine, 2006
- Real-Time PCR for mRNA QuantitationBioTechniques, 2005
- Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong?BMC Molecular Biology, 2005
- Real-time RT-PCR normalisation; strategies and considerationsGenes & Immunity, 2005
- Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applicationsNucleic Acids Research, 2004
- Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysisNucleic Acids Research, 2003
- Cytokine gene expression in monocytes of patients undergoing cardiopulmonary bypass surgery evaluated by real‐time PCRJournal of Cellular and Molecular Medicine, 2003
- Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) dataNeuroscience Letters, 2003
- Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problemsJournal of Molecular Endocrinology, 2002
- Validation of a quantitative method for real time PCR kineticsBiochemical and Biophysical Research Communications, 2002