Virus-Free Friend Virus-Induced Tumor: In Vitro and In Vivo Characteristics23

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Abstract
An ascites tumor variant of a friend virus-induced tumor was grown in tissue culture. The cells grew rapidly in vitro and characteristically remained suspended in the medium. Morphologically, they closely resembled the tumor cells grown in vivo and, when transplanted back into mice, produced reticulum cell sarcomas indistinguishable from those of the original tumor line. After 32 passages in tissue culture, friend virus could no longer be detected in the cells, nor could it be isolated from serially transplanted tumors during 10 subsequent passages in mice. The average number of cells required to produce tumors was lower for the virus-free tumor cells than the original tumor which was rich in virus. It is concluded that the virus plays little part in determination of either transplantability or virulence of the tumor, once the original transformation has occurred.

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