Priming of human neutrophil superoxide generation by tumour necrosis factor‐α is signalled by enhanced phosphatidylinositol 3,4,5‐trisphosphate but not inositol 1,4,5‐trisphosphate accumulation

Publisher Website
Google Scholar
Abstract
In human neutrophils, significant agonist-stimulated superoxide anion (O− 2) release is observed only after exposure to a priming agent such as TNFα. We have investigated the potential for TNFα to modulate N-formyl-Met-Leu-Phe (fMLP)-triggered Ins(1,4,5)P3 and PtdIns(3,4,5)P3 accumulation. TNFα pretreatment did not affect basal or stimulated Ins(1,4,5)P3 levels but greatly upregulated fMLP-stimulated PtdIns(3,4,5)P3 accumulation, in a manner that matched, both temporally and in magnitude, the increase in O− 2 generation implying a possible role for PtdIns(3,4,5)P3 in signalling primed O− 2 release.