Spectrophotometric Method for Measurement of Lactose in Milk and Milk Products by Using Dialysis

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Abstract
A simple method for measuring lactose in milk and milk products by using dialysis is described. A sample of milk, diluted milk powder, cultured buttermilk, or Cheddar cheese whey is dialyzed against water in a test tube at 45°C for 1.5 h. Lactose from the product is extracted into the water and Teles reagent is added. Absorbance was converted to percent lactose using a standard curve. Lactose obtained by the dialysis method was comparable to results obtained by the ultraviolet enzymatic and infrared methods. The method was not suitable for yogurt, which contains galactose that reacts with the Teles reagent. The new method for lactose measurement is simple and does not require precipitation of proteins, filtration, or centrifugation.

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