Measurement of K vitamins in human and animal plasma by high-performance liquid chromatography with fluorometric detection.

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Abstract
A sensitive method for measuring endogenous vitamin K1 (VK1) and vitamin K2 (MK-n) in human and animal plasma was developed based on high-performance liquid chromatography combined with coulometric reduction and fluorometric detection. The vitamins were extracted from plasma, purified with a silica Sep-Pak cartridge, and then measured by using the standard method. The detection limits of VK1 and MK-4 were 5 pg/injection for each of the standard substances and 50 pg/ml for each of the two as components of human plasma.