Inhibition of telomerase activity by a cell‐penetrating peptide nucleic acid construct in human melanoma cells
Open Access
- 9 May 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 473 (2) , 241-248
- https://doi.org/10.1016/s0014-5793(00)01540-4
Abstract
We investigated the effect of two peptide nucleic acids (PNAs), which are complementary to the RNA component of human telomerase, on the catalytic activity of the enzyme. PNAs induced a dose‐dependent reduction of telomerase activity in cell extracts from human melanoma cell lines and surgical specimens. To down‐regulate telomerase in intact cells, we generated a chimeric molecule synthesized by coupling the 13‐mer PNA to the Antennapedia peptide. The PNA construct induced a dose‐ and time‐dependent inhibition of telomerase activity. However, a 20‐day exposure to the PNA construct only caused a slight increase in melanoma cell doubling time and failed to induce any telomere shortening.Keywords
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