Identification and quantification of rodent malaria strains and species using gene probes
- 1 January 1992
- journal article
- research article
- Published by Cambridge University Press (CUP) in Parasitology
- Vol. 105 (1) , 21-27
- https://doi.org/10.1017/s0031182000073649
Abstract
A DNA probe PCsv4 and a subclone thereof PCsv4.1, hybridize specifically to rodent malaria DNA. DNA purified from a small volume (10 μ1) of infected mouse blood was used to determine the composition of the parasite population present. The hybridization signal following PCsv4 probing of slot-blotted DNA correlated directly with parasitaemia. The hybridization pattern and Intensity, resulting from probing restriction enzyme digested and Southern-blotted genomic DNA, determined the identity of the infecting parasite line(s), and provided a semi-quantitative measure of parasite burden. Fifteen parasite lines representative of all fourPlasmodiumspecies infecting rodents can be differentiated in this way.Keywords
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