Crystal structure of trichosanthin-NADPH complex at 1.7Å resolution reveals active-site architecture
- 1 October 1994
- journal article
- Published by Springer Nature in Nature Structural & Molecular Biology
- Vol. 1 (10) , 695-700
- https://doi.org/10.1038/nsb1094-695
Abstract
We describe here the crystal structure of the trichosanthin-NADPH complex determined at a resolution of 1.7 A. The adenine base stacks between Tyr 70 and Tyr 111. Arg 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and, through a water molecule, to N(9) of adenosine, respectively. This is the first high resolution structure of a complex between a ribosome-inactivating protein and a substrate analogue, in which the electron density of the N-glycosidic bond is well defined and the preassociated water, thought to be responsible for hydrolyzing the N-C bond, is also explicitly elucidated.Keywords
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