Detection of cell-cycle stage by fluorescence in situ hybridization: its application in human interphase cytogenetics
- 1 January 1992
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 61 (2) , 91-94
- https://doi.org/10.1159/000133378
Abstract
Distinct cell-cycle-dependent changes in the conformation of centromeric chromatin in a specific human chromosome containing α-satellite DNA have been demonstrated by fluorescence in situ hybridization (FISH). This method, based upon specific FISH signal morphology, allows simultaneous analysis of chromosomal aneuploidy and detection of specific cell-cycle stage(s) of human tumor and/or normal cell populations in a single preparation of interphase cells. This interphase cytogenetic procedure might prove useful for both basic and clinical research involving human cells.Keywords
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