Applicability of DNA isolated from syncytiotrophoblast vesicles to gene amplification and molecular analysis
- 1 May 1993
- journal article
- Published by Wiley in Prenatal Diagnosis
- Vol. 13 (5) , 335-340
- https://doi.org/10.1002/pd.1970130504
Abstract
Maternal contamination of fetal DNA represents a major problem when highly sensitive molecular techniques are used in the prenatal diagnosis of genetic diseases. For this reason, we have studied the possibility of using DNA isolated from syncytiotrophoblast vesicles as a target of gene amplification (PCR). Three PCR systems were selected which included a repetitive 149 bp fragment of the Y chromosome, the VNTR locus D1S80, and a portion of the β‐globin gene. The results of these experiments indicate that DNA isolated from syncytiotrophoblast vesicles is free of maternal contamination and is suitable for gene amplification and DNA analysis.Keywords
This publication has 6 references indexed in Scilit:
- Long-term T-cell reconstitution after hematopoietic stem-cell transplantation in primary T-cell–immunodeficient patients is associated with myeloid chimerism and possibly the primary disease phenotypeBlood, 2007
- Rapid detection and prenatal diagnosis of β-thalassaemia: studies in Indian and Cypriot populations in the UKThe Lancet, 1990
- DETECTING MATERNAL CELL CONTAMINATION IN PRENATAL DIAGNOSISThe Lancet, 1989
- Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplificationNucleic Acids Research, 1988
- An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA SequencesNew England Journal of Medicine, 1987
- Efficient direct chromosome analyses and enzyme determinations from chorionic villi samples in the first trimester of pregnancyHuman Genetics, 1983