INVITRO EFFECT OF MEDIUM SIZE MOLECULES ON LYMPHOCYTE-PROLIFERATION

  • 1 January 1976
    • journal article
    • research article
    • Vol. 24  (3) , 189-193
Abstract
Eluates from [human] uremic sera were obtained by chromatography on Sephadex G-25. They were assayed on lymphocytes cultured in the presence of various stimulants. Lymphocyte proliferation induced by mitogens or allogeneic cells was markedly inhibited in the presence of medium size molecules (MM), or of larger molecules (MW > 5000). E [erythrocyte]-rosette formation was not significantly affected by any eluate fraction obtained from uremic sera. Molecules with a MW > 5000 were cytotoxic for lymphocytes, but MM were not. The mechanism of action of MM on the inhibition of lymphocyte proliferation in vitro is still to be determined. These molecules could play a significant part in the in vivo cell-mediated immunodeficiency secondary to renal failure.

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