Endogenous VH gene family expression in immunoglobulin-transgenic mice: evidence for selection of antibody repertoires
- 1 January 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 3 (1) , 67-73
- https://doi.org/10.1093/intimm/3.1.67
Abstract
VH gene family expression in single cells of the emergent, available and actual B cell repertoires of C57BL/6 mice was compared to that of two immunoglobulln (lg)-transgenic B6 lines (B6-Sp6 and M54). We found that 50% of which express endogenous VH genes. The pattern of endogenous VH gene family expression in the actual repertoire of B6-Sp6 mice is indistinguishable from that of normal B6 mice. In contrast, actual repertoires of M54 mice differ by a 4- to 5-fold higher representation of the VHQ52 family. These results demonstrate a powerful positive selection of B cells into the secretory compartments of unimmunized animals, show that actual and available repertoires differ very markedly, and suggest that V region interactions participate in the selection of ‘natural antibody’ repertoires.Keywords
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