Yeast Two-Hybrid Systems and Protein Interaction Mapping Projects for Yeast and Worm

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Abstract
The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as functional genomics. One approach to investigate the properties of a proteome of interest is by systematic analysis of proteinprotein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential proteinprotein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans.
Funding Information
  • National Cancer Institute (1 R21 CA81658 A 01, 1 RO1 HG01715-01)