Dependence of 4'-(hydroxymethyl)-4,5',8-trimethylpsoralen photoaddition on the conformation of RNA
- 16 March 1982
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 21 (6) , 1363-1368
- https://doi.org/10.1021/bi00535a040
Abstract
The photoaddition of 4''-(hydroxymethyl)-4,5'',8-trimethylpsoralen (HMT) to different conformational states of RNA was studied. Poly(U), poly(A,U) (random copolymer), poly(A-U) (alternating copolymer), poly(A).cntdot.poly(U) (double stranded), and poly(U).cntdot.poly(A).cntdot.poly(U) (triple stranded) were reacted with HMT at different temperatures and salt concentrations. The conformation of the polymers was monitored by UV absorption and circular dichroism. The rate of HMT photoaddition changed dramatically at structural transitions in the RNA. The alternating copolymer poly(A-U) had the highest rate of addition. Low salt and temperature produced maximal incorporation.This publication has 16 references indexed in Scilit:
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