Improved procedure and colorimetric test for the detection of ortho- and meta-cleavage of protocatechuate by Pseudomonas isolates
- 1 July 1974
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 20 (7) , 1059-1061
- https://doi.org/10.1139/m74-163
Abstract
A sensitive and clear-cut method for the demonstration of ortho- and meta-cleavage of protocatechuate is described. Pseudomonas strains to be tested for the mode of ring fission should be grown on a synthetic medium containing p-hydroxybenzoate, quinate, or glucose–yeast extract as sole carbon source. Suspensions of these organisms are tested for ring-fission mechanism to detect the constitutive or inducible nature of protocatechuate 3,4-oxygenase.Keywords
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