Interaction between Serratia Protease and Human Plasma α2Macroglobulin
- 1 April 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 89 (4) , 1231-1237
- https://doi.org/10.1093/oxfordjournals.jbchem.a133308
Abstract
Serratia protease (TSP) [EC 3.4.24] was bound stoichiometrically to α2macroglobulin (α2M), which was purified and crystallized from human plasma, but apo TSP was not bound. On formation of the TSP-α2M complex the enzymatic activity of the bound TSP was affected with respect to substrates; Km values of the bound TSP were unchanged but Vmax values were reduced. α2M was cleft at the mid-region of its subunits chains by TSP, which resulted in a conformational change of the α2M molecule with TSP.This publication has 5 references indexed in Scilit:
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