SYNTHESIS AND BIOLOGICAL ACTIVITY OF OVINE β-LIPOTROPIN-(41-91)-HENKAIPENTEKONTAPEPTIDE
- 12 January 2009
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 11 (2) , 179-184
- https://doi.org/10.1111/j.1399-3011.1978.tb02837.x
Abstract
The 51-residue peptide ovine .beta.-lipotropin-(41-91) was synthesized by the solid-phase method in about 5% overall yield. The synthetic product was characterized by partition chromatography on agarose gel, TLC in 2 solvent systems, paper electrophoresis at 2 pH values, polyacrylamide gel electrophoresis, amino acid analyses of acid and enzymic hydrolysates, and bioassay for lipolytic and melanotropic activities. The synthetic peptide is about 5.4 times as active on a weight basis as ovine .beta.-lipotropin in the lipolytic assay [rabbit fat cells]. In the melanotropic assay [frog skin], it was about 2.4 times more active than the .beta.-lipotropin but only 5% as active as bovine .beta.-melanotropin. It had negligible opiate activity in the guinea pig ileum assay.Keywords
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