Production of 3α-Androstanediol Glucuronide in Human Genital Skin*

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Abstract
3α-Androstanediol glucuronide (3αdiol-G) is produced extrasplanchnically and is a good clinical marker of androgen action in peripheral tissues. However, the direct formation of androgen glucronides in peripheral sites such as skin has not been determined in man. Genital skin from 21 premenopausal women and 8 men and foreskin from 6 neonates were incubated with either [14C]testosterone or [14C]dihydrotestosterone (DHT) to determine the production of DHT glucuronide and 3αdiol-G in skin. After hydrolysis of incubation medium with glucuronidase, followed by extraction and sequential chromatography, constant 3H to 14C ratios of 3αdiol confirmed the production of 3αdiol-G by skin. In addition, high pressure liquid chromatographic separation of the medium confirmed the direct production of DHT glucuronide and 3αdiol-G. The conversion of DHT to 3αdiol-G was higher than the conversion from testosterone (P < 0.05), and conversion was higher in men than in women. These data provide evidence for the direct formation of C19 steroid glucuronides by human skin.

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