Determination of Serum Lipoproteins in Clinically Healthy Iranian Crossbred Cattle by Agarose Gel Electrophoresis

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Abstract
Nazifi, S., Saeb, M. and Rowghani, E. 2003. Determination of serum lipoproteins in clinically healthy Iranian crossbred cattle by agarose gel electrophoresis. J. Appl. Anim. Res., 23: 59–64. To determine the lipoprotein components of the serum, blood samples were collected from the jugular vein of 100 clinically healthy Iranian crossbred cattle according to their age (5 years) and sex. Separation on agarose gel electrophoresis revealed four separate serum lipoproteins, viz., α-lipoprotein (67.53±1.16%), pre-β-lipoprotein (14.9±0.80%), β-lipoprotein (17, 25±0.37%) and chylomicron (0.26±0.03%). The normal concentrations of serum cholesterol and triglyceride were 3.80±0.11 and 0.38±0.02 mmol/l, respectively. Age was significantly and directly correlated with cholesterol, triglyceride, α-lipoprotein (HDL), pre-β-lipoprotein (VLDL) and chylomicron concentrations. Sex had no effect on parameters studied.