Cloning and Sequence Analysis of a Plasmid-encoded 2-Haloacid Dehalogenase Gene fromPseudomonas putidaNo. 109
- 1 January 1994
- journal article
- Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry
- Vol. 58 (1) , 160-163
- https://doi.org/10.1271/bbb.58.160
Abstract
The 2-haloacid dehalogenase of Pseudomonas putida No. 109 was mediated by a 74-kb conjugative plasmid, which was transferred by mating into Pseudomonas and Escherichia coli and there expressed the dehalogenase. A 2.8-kb EcoRI-fragment generated from the plasmid was cloned and sequenced. The dehalogenase gene (dehH109) was identified by comparison with the N-terminal amino acid sequence and the molecular weight of the enzyme protein. The gene dehH109 coded for a 224-amino acid protein of Mr 25,231, which showed significant homology to the other four l-specific 2-haloacid dehalogenases from Pseudomonas sp. CBS3, P. putida AJ1, and Xanthobacter autotrophics GJ10 and also to the haloacetate dehalogenase H-2 from Moraxella sp. strain B, but no homology with another haloacetate dehalogenase H-1 and the d-specific 2-haloacid dehalogenase from P. putida AJ1.Keywords
This publication has 0 references indexed in Scilit: